Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 1 AR-derived Tfhs in a DC co-culture system promote DC maturation. (A) Gating strategy and flow plot for Tfhs sorting according to Materials and Methods. (B) A schematic of Tfhs/DCs Transwell co-culture system. (C) Relative mRNA expression levels of CD80 or CD86 in treated or untreated DCs are expressed as mean ± standard error from at least three independent experiments. *** P < 0.001; **** P < 0.0001. (D) Flow cytometry results of DC cell surface expression of the co-stimulatory molecules CD80, CD86 and MHCII in control (no treatment), WT mice-derived Tfhs, AR mice-derived Tfhs, or LPS treated group. Flow cytometry results demonstrate that the ratio of CD80, CD86 and MHC II of DCs was significantly increased in the AR-derived Tfhs/DCs co-culture group. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Co-Culture Assay, Expressing, Flow Cytometry, Control

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 2 Expression of CD80 and CD86 of DCs under four different conditions. Immunofluorescence staining was performed to determine the expression level of CD80 and CD86 on DC cell surface. Scale bar at the bottom right stands for 50 μm. Data shown are representative of 3 independent experiments. Green represents CD80 staining, red CD86 staining, and blue DAPI staining for cell nuclei.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 3 Tfhs secrete exosomes and AR-derived Tfh exosomes promote DC maturation. (A) Morphology of exosomes isolated from WTor AR mice by scanning electron microscopy. Scale bar represents 100 nm. (B) Comparison of relative mRNA expression level (mean ± standard error of at least three independent experiments) of CD80 and CD86 in the DCs treated with control (no treatment), WT mice-derived Tfh exosome, AR mice-derived Tfh exosome, LPS, or WT mice-derived Tfh exosomes with the addition of GW4869. *** P < 0.001; **** P < 0.0001. (C) Flow cytometry results of DC cell surface expression of the co-stimulatory molecules CD80, CD86 and MHCII in control (no treatment), WT mice-derived Tfhs exosome, AR mice-derived Tfhs exosome group, LPS, or WT mice-derived Tfh exosomes with the addition of GW4869 group. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Isolation, Electron Microscopy, Comparison, Expressing, Control, Flow Cytometry

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 4 Expression of CD80 and CD86 on DC cells under five different conditions. Sections of DCs were stained Immunofluorescence dyes for CD80 (green) and CD86 (red). DAPI was used to stain nuclei (blue). Representative images are shown from at least three independent experiments. Scale bar at the bottom right stands for 50 μm.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Expressing, Staining, Immunofluorescence

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 5 miR-142-5p in AR mouse-derived exosomes regulated DC maturation. (A) Heat map of differentially expressed miRNAs. The heat map was divided into two columns (left: AR; right: Control). Up- or down-regulation of the miRNAs was represented with red or green color, respectively. A specific miRNA, miR-142b, was down- regulated in AR, as indicated with a yellow arrow. (B) The expression level of miR-142-5p in exosomes of Tfhs from AR vs control mice. (C) Detection of the expression level of CD80 and CD86 in different groups with overexpression or inhibition of miR-142-5p. *** P < 0.001; **** P < 0.0001. (D) Flow cytometry analysis of co-stimulatory molecules CD80, CD86 and MHCII on surface of DCs transfected with miR-142-5p mimics or inhibitors. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Control, Expressing, Over Expression, Inhibition, Flow Cytometry, Transfection

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 6 miR-142-5p targets CDK5 to mediate the regulation of DC maturation by Tfhs derived from AR mice. (A) The potential miR-142-5p target sites in CDK5 transcript. (B) Levels of relative luciferase activity in HEK293T cells following transfection with PGL-3-control-WT-miR-142-5p mimics, PGL-3-CDK5-WT-miR-142-5p mimics and PGL-3-CDK5-MT-miR-142-5p mimics respectively (1 μg). The relative luciferase activity refers to the ratio of firefly luminescence/renilla luminescence. (C) Relative expression of miR-142-5p after CDK5 knockdown. (D) CDK5 expression level under overexpression or inhibition of miR-142-5p by RT-qPCR. The values shown are mean ± SD of three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. (E) CDK5 expression level under overexpression or inhibition of miR-142-5p by Western Blot. (F) Flow cytometry assesses the CD80, CD86 and MHCII expression on the surface of DCs by inhibiting CDK5. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Luciferase, Activity Assay, Transfection, Control, Expressing, Knockdown, Over Expression, Inhibition, Quantitative RT-PCR, Western Blot, Flow Cytometry

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 7 Tfh-derived exosomes promote DC cell maturation through miR-142-5p/CDK5/STAT3 pathway. (A) Relative expression of miR-142-5p and CDK5 in DCs upon LPS stimulation at different time points. The values shown are mean ± SD of three independent experiments. ** P < 0.01; **** P < 0.0001. (B) CDK5 expression level changed with prolonged LPS stimulation. (C) Detection of STAT3 phosphorylation in overexpression and inhibition of miR-142-5p. (D) Detection of STAT3 phosphorylation in inhibition of miR-142-5p with or without si-CDK5. (E) Expression of CD80 and CD86 on DCs with STAT activator or STAT inhibitor and LPS stimulation for different times. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: Derivative Assay, Expressing, Phospho-proteomics, Over Expression, Inhibition

Journal: Journal of Inflammation Research

Article Title: Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway

doi: 10.2147/jir.s365217

Figure Lengend Snippet: Figure 8 Results from an in vivo experiment showing the effect of AR-derived Tfh exosomes on occurrence of AR. (A) Hematoxylin and eosin staining of nasal mucosal from different group of mice. Scale bars at the bottom right of each image represent 50 µm. The area of mucosal thickening and inflammation appeared as dark blue area in the image, with one area indicated with a yellow arrow. (B) The relative mRNA expression of CD80 and CD86 in mouse nasal mucosa of various groups. The data shown are mean ± standard error of at least 3 independent experiments. (C) Flow cytometry results of the co-stimulatory molecules CD80, CD86 and MHCII in DCs. Dot plots with the label for the proportion of CD80+ CD86+ or MHCII+ cells are representative of at least three independent experiments. (D) Inflammatory factors including IFN-γ, IgE, IL-13 and IL-17 are studied in the serum of mice of various group. ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Rabbit polyclonal anti-STAT3 antibody (Cat# A19566, 1:1000 diluted for WB), anti-phospho-STAT3-Y705 antibody (Cat# AP0705, 1:1000 diluted for Western blot), rabbit anti-CD80 antibody (Cat# A16039, 1:100 diluted for immunofluorescence, 1:2000 diluted for Western blot) and rabbit anti-CD86 antibody (Cat# A1199, 1:100 diluted for immunofluorescence, 1:1000 diluted for Western blot) were purchased from Abclonal (Wuhan, Hubei, China).

Techniques: In Vivo, Derivative Assay, Staining, Expressing, Flow Cytometry

Journal: Bioengineering & translational medicine

Article Title: Plasma-activated medium triggers immunomodulation and autophagic activity for periodontal regeneration.

doi: 10.1002/btm2.10528

Figure Lengend Snippet: FIGURE 2 PAM reduced the expression of M1 markers in an inflammatory microenvironment and promoted M1 to M2 phenotypic shift of macrophages. (A) The gene expression levels of inflammatory macrophage markers after PAM treatment were displayed in a box plot graph. The data from five independent experiments were shown as mean ± SD (*p < 0.05, one-way ANOVA); (B) the concentrations of inflammatory cytokines derived from PAM-stimulated macrophages are shown. The results of five independent experiments are presented as mean ± SD (*p < 0.05, one-way ANOVA); (C) the IF staining images show the change of M1/M2 ratio after PAM treatment (scale bars: 5 μm). Data from five randomly selected field of view (FOV) were analyzed using the ImageJ software to determine the intensity of CD86 and CD206. The results are presented as mean ± SD (*p < 0.05, one-way ANOVA).

Article Snippet: The cells were then incubated with the mouse polyclonal antibody against CD86 (1:100, sc-28347, Santa Cruz Biotechnology), the rabbit polyclonal antibody against CD206 (1:200, ab64693, Abcam), and the rabbit monoclonal antibody against iNOS (1:100, 13120S, Cell Signaling) overnight at 4 C. The secondary antibodies used were goat antimouse immunoglobulin G (IgG) Alexa Fluor 488 (1:100, A-11001, Life Technologies) and goat anti-rabbit IgG Alexa Fluor 568 (1:200, A-11036, Life Technologies).

Techniques: Expressing, Gene Expression, Derivative Assay, Staining, Software

Journal: Experimental and Therapeutic Medicine

Article Title: Distinct phenotypic expression levels of macrophages in neonatal lungs

doi: 10.3892/etm.2021.9800

Figure Lengend Snippet: Characterization of macrophage phenotypes in rats at different ages. (A) Expression levels of M1 and M2 markers in the neonatal, adult and aged lung tissues. iNOS and CD86 are markers for M1; Arg-1 and CD206 are markers for M2. * P<0.05 vs. adults (Kruskal-Wallis; n=5). (B) Upper quadrants of flow cytometry diagrams represent the amount of circulating blood cells that co-expressed the M1 marker CD86 (x-axis) and monocyte marker CD68 (y-axis). Representative histograms for isotype controls for CD86 and CD68 staining in total white blood cells (lower left panel). The percentage of CD86 + /CD68 + cells in the peripheral blood was significantly higher in neonates than adults. * P=0.007 vs. neonates, assessed using an independent t-test (adult, n=3; neonate, n=9). iNOS, inducible nitric oxide synthase; Arg, arginase.

Article Snippet: The primary antibodies used were: iNOS (mouse monoclonal antibody; cat. no. 610432; BD Transduction Laboratories; BD Biosciences), CD86 (rabbit polyclonal antibody; cat. no. GTX 34569; GeneTex, Inc.), arginase (Arg)-1 (rabbit polyclonal antibody; cat. no. 16001-1-AP; ProteinTech Group, Inc.), CD206 (rabbit polyclonal antibody; cat. no. 18704-1-AP; ProteinTech Group, Inc.), NF-κB (rabbit polyclonal antibody; cat. no. 622602; BioLegend, Inc.) and b-actin (mouse monoclonal antibody; cat. no. MAB 1501; Sigma-Aldrich; Merck KGaA). iNOS and CD86 were defined as cell markers for M1 macrophages; Arg-1 and CD206 were defined as cell markers for M2 macrophages ( , ).

Techniques: Expressing, Flow Cytometry, Marker, Staining

Journal: Nature Communications

Article Title: A photo-triggered self-accelerated nanoplatform for multifunctional image-guided combination cancer immunotherapy

doi: 10.1038/s41467-023-40996-2

Figure Lengend Snippet: a Microscopic images of RAW264.7 cells before and after treating with LPS and IFN-γ. Scale bars: 20 μm. b Representative flow cytometry plots of M1 phenotype (CD80 + CD86 + ) in RAW264.7 cells before and after treating with LPS and IFN-γ. Representative DLS results and (inset) TEM images of c MPNPs and d M1-MPNPs. Scale bars: 100 nm. e , f Average sizes and zeta potentials of various NPs. Data are presented as mean ± SD ( n = 3 independent experiments). g Representative western blots of CD86 and iNOS expression in different formulations. h The excitation-emission mapping of M1-MPNPs. For b – d , g , h , experiment was repeated three times independently with similar results. Source data are provided as a Source Data file.

Article Snippet: Antibodies used: Western blotting: Anti-CD86 (C-terminal) Polyclonal antibody (1:1000, Proteintech, Rabbit mAb, #26903-1-AP); Recombinant Anti-iNOS antibody (1:1000, Abcam, Rabbit mAb, #ab178945); CD47 (D3O7P) Rabbit mAb (1:1000, Cell Signaling Technology, #63000); Na,K-ATPase Antibody (1:1000, Cell Signaling Technology, #3010); Integrin alpha 4/CD49D (1:1000, Abcam, Rabbit mAb, #ab81280); Integrin beta 1 (1:1000, Abcam, Rabbit mAb, #ab52971).

Techniques: Flow Cytometry, Western Blot, Expressing

Journal: Nature Communications

Article Title: A photo-triggered self-accelerated nanoplatform for multifunctional image-guided combination cancer immunotherapy

doi: 10.1038/s41467-023-40996-2

Figure Lengend Snippet: a Experimental outline showing the treatment steps and procedures for evaluating the therapeutic outcomes in 4T1 tumor-bearing mice. The “i.v.” represents “intravenous injection”. The illustration was created with BioRender.com. Plots of b tumor volume, c individual tumor volume, d tumor weight, and e body weight of the 4T1 tumor-bearing mice with various treatments. Data are presented as mean ± SD ( n = 5 mice). Statistical significance was determined using one-way ANOVA. f Representative H&E staining and TUNEL staining of tumor sections harvested from the mice receiving different treatments on day 14 ( n = 3 mice). Scale bars: 50 μm. Experiment was repeated three times independently with similar results. g Representative CLSM images showing ecto-CRT expression (red pseudocolor) on the tumor sections from the mice receiving various treatments. Scale bars: 50 μm. Experiment was repeated three times independently with similar results. h Quantitative data of the population of DC maturation (CD11c + CD80 + CD86 + ) in lymph nodes after various treatments. Data are presented as mean ± SD ( n = 3 mice). Statistical significance was determined using one-way ANOVA. i Quantitative data of the percentages of CD8 + T cells in tumor. Data are presented as mean ± SD ( n = 3 mice). Statistical significance was determined using one-way ANOVA. Source data are provided as a Source Data file.

Article Snippet: Antibodies used: Western blotting: Anti-CD86 (C-terminal) Polyclonal antibody (1:1000, Proteintech, Rabbit mAb, #26903-1-AP); Recombinant Anti-iNOS antibody (1:1000, Abcam, Rabbit mAb, #ab178945); CD47 (D3O7P) Rabbit mAb (1:1000, Cell Signaling Technology, #63000); Na,K-ATPase Antibody (1:1000, Cell Signaling Technology, #3010); Integrin alpha 4/CD49D (1:1000, Abcam, Rabbit mAb, #ab81280); Integrin beta 1 (1:1000, Abcam, Rabbit mAb, #ab52971).

Techniques: Injection, Staining, TUNEL Assay, Expressing

Journal: Nature Communications

Article Title: A photo-triggered self-accelerated nanoplatform for multifunctional image-guided combination cancer immunotherapy

doi: 10.1038/s41467-023-40996-2

Figure Lengend Snippet: a Schematic illustration of the procedure for evaluation of the antitumor effect and immune responses induced by various treatments in bilateral 4T1 tumor-bearing mice model. The “i.v.” represents “intravenous injection”. The illustration was created with BioRender.com. Plots of b primary tumor volume, c distant tumor volume, d individual tumor growth curve of distant tumor, and e body weight of the bilateral 4T1 tumor-bearing mice post various treatments. Data are presented as mean ± SD ( n = 5 mice). f , g Representative flow cytometry analysis and quantitative data of the population of DC maturation (CD11c + CD80 + CD86 + ) in lymph nodes after various treatments on day 14. Data are presented as mean ± SD ( n = 3 mice). Statistical significance was determined using one-way ANOVA. h Quantitative data of the percentages of CD8 + T cells in primary tumors collected from bilateral 4T1 tumor-bearing mice after various treatments on day 14. Data are presented as mean ± SD ( n = 3 mice). Statistical significance was determined using one-way ANOVA. i , j Representative flow cytometry plots and quantitative data of the percentages of CD8 + T cells in distant tumors collected from bilateral 4T1 tumor-bearing mice after various treatments on day 14. Data are presented as mean ± SD ( n = 3 mice). Statistical significance was determined using one-way ANOVA. Representative flow cytometry data of CD8 + Tem cells (CD44 + and CD62L ‒ ) in spleen, H&E and TUNEL staining of distant tumors in bilateral 4T1 tumor-bearing mice from k “PBS” group and l “M1-MPNPs + L” group on day 14 ( n = 3 mice). Scale bars: 50 μm. Experiment was repeated three times independently with similar results. Source data are provided as a Source Data file.

Article Snippet: Antibodies used: Western blotting: Anti-CD86 (C-terminal) Polyclonal antibody (1:1000, Proteintech, Rabbit mAb, #26903-1-AP); Recombinant Anti-iNOS antibody (1:1000, Abcam, Rabbit mAb, #ab178945); CD47 (D3O7P) Rabbit mAb (1:1000, Cell Signaling Technology, #63000); Na,K-ATPase Antibody (1:1000, Cell Signaling Technology, #3010); Integrin alpha 4/CD49D (1:1000, Abcam, Rabbit mAb, #ab81280); Integrin beta 1 (1:1000, Abcam, Rabbit mAb, #ab52971).

Techniques: Injection, Flow Cytometry, TUNEL Assay, Staining

Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a , b TEM images of P-CD@MM. c Measurements of the expression levels of CD86 and CD206 in each group. d Representative fluorescence images of cellular uptake of P-CD@MM in 4T1 or NIH-3T3. e , f Relative cell viability of NIH-3T3 and 4T1 cells after different treatments ( n = 6 independent samples). g , h Live/dead cell and ROS staining of 4T1 cells after different treatments. For ( a – d , g , h ), experiment was repeated three times independently with similar results. Data are presented as mean ± SD and statistical significance was analyzed via two-tailed Student’s t test. Source data are provided as a file.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Expressing, Fluorescence, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a Confocal images of CRT exposure in 4T1 cells after different treatments. b , c Evaluation of HMGB1 and ATP levels in 4T1 cells after different treatments ( n = 6 independent samples). d A scheme to show the experimental process of the evaluation of DC maturation (Relative drawing materials were created in BioRender. zhenlin, Z. (2026) https://BioRender.com/9u519d6 ). e , f Evaluation of DC maturation level in each group ( n = 6 mice). g – i Flow cytometry analysis and the corresponding quantitative results of CD86 + and CD206 + expression in TAMs of BMDM after different treatments ( n = 6 mice). For ( a ), experiment was repeated three times independently with similar results. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA. Source data are provided as a file.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Flow Cytometry, Expressing

Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a CRT staining of tumor tissues after different treatments ( n = 3 mice). b Expression levels of CD4 + T cells and CD8 + T cells in the primary tumors after different treatments ( n = 6 mice). c Expression levels of CD86 + and CD206 + in TAMs in the primary tumors after different treatments ( n = 6 mice). d Schematic diagram of P-CD@MM-mediated PDT to induce an immune response in vivo (Relative drawing materials were created in BioRender. zhenlin, Z. (2026) https://BioRender.com/9u519d6 ). For ( a ), experiment was repeated three times independently with similar results.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Staining, Expressing, In Vivo

Journal: Cardiovascular Diagnosis and Therapy

Article Title: Establishment of a medial arterial calcification model in C57BL/6J mice via arterial intimal injury

doi: 10.21037/cdt-2025-435

Figure Lengend Snippet: Pro-inflammatory milieu in calcified arteries and the impact of guide wires with different diameters on MAC model generation. (A) Representative IF staining results showed that CD68 (green color) and CD86 (red color) expression was low in the mice from the sham operation group and significantly higher in the mice from the experimental group in terms of the fluorescence density and intensity. Individual channels are presented separately for clarity. Nuclei were counterstained with DAPI (not shown). (B) Representative IHC staining results showed that the IL-1β and IL-6 expression in the calcified blood vessel from the experimental group was significantly higher than that in the sham operation group, with slightly higher intensity. (C) Incidence rates of ossification caused by the 0.35, 0.40, and 0.45 mm diameter guide wires. (D) According to the MAC histopathological grading criteria , the MAC grading ratios for the MAC caused by the 0.35, 0.40, and 0.45 mm diameter guide wires in the mouse MAC. Sample sizes were: n=10 for Sham group, n=12 for 0.35 mm group, and n=13 for 0.40 mm group, n=15 for 0.45 mm group. (E) Image of rough guide wires with diameters of 0.35, 0.40, or 0.45 mm. DAPI, 4',6-diamidino-2-phenylindole; IF, immunofluorescent; IHC, immunohistochemistry; IL-1β, Interleukin-1 beta; IL-6, Interleukin-6; MAC, medial arterial calcification.

Article Snippet: Subsequently, samples were incubated with rabbit anti-CD86 polyclonal antibody (1:100; Cat #19589; Cell Signaling Technology, Danvers, USA) diluted in 1% BSA/PBS at 4 °C overnight.

Techniques: Staining, Expressing, Fluorescence, Immunohistochemistry